Single-Dose Assay Technique for Variola Virus12
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منابع مشابه
Single-Dose Assay Technique for Variola
RILEY, JEAN M. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), AND MICHAEL D. ORLANDO. Singledose assay technique for variola virus. Appl. Microbiol. 12:7-9. 1964.-A biological assay for variola virus was needed that would (i) require a minimum of time and (ii) have acceptable precision. Preliminary titrations made in 11-day-old embryonated eggs and in suckling mice (6 to 24 ...
متن کاملSingle - Dose Assay Technique for Variola Virus " 2
RILEY, JEAN M. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), AND MICHAEL D. ORLANDO. Singledose assay technique for variola virus. Appl. Microbiol. 12:7-9. 1964.-A biological assay for variola virus was needed that would (i) require a minimum of time and (ii) have acceptable precision. Preliminary titrations made in 11-day-old embryonated eggs and in suckling mice (6 to 24 ...
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A quantitative assay for infective variola virus particles was developed which is based on the enumeration of cells containing fluorescent viral antigen after infection of McCoy cell monolayers. The direct fluorescent-antibody technique was employed to stain cells. The efficiency of virus adsorption was markedly enhanced by centrifugation of virus inoculum onto McCoy cell monolayers at 500 x g ...
متن کاملApplication of the single-dose assay technique to psittacosis.
When suspensions of psittacosis organisms were injected intracerebrally into groups of mice, a nearly linear relationship was observed between the concentration of the organisms injected and the mean time to death of the mice. Thirty-four psittacosis preparations were assayed and, by plotting the relationship between the reciprocal time to death for mice given the 10(-3) dilution of agent again...
متن کامل120 The Infectious Dose of Variola ( Smallpox ) Virus
Quantitative estimation of an individual's risk of infection due to airborne pathogens requires knowledge of the pathogen's infectious dose, in addition to estimates of the pathogen's airborne concentration and the person's exposure duration. Based on our review of the published literature on poxvirus infection, we conclude that the infectious dose of variola (smallpox) virus is likely one viru...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1964
ISSN: 0003-6919
DOI: 10.1128/aem.12.1.7-9.1964